Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IRF3 in HeLa, A-431, HepG2, and NIH/3T3 cells. DyLight 488™ is a registered trademark of Thermo Fisher Scientific.
Immunoprecipitation Analysis: A representative lot immunoprecipitated IRF3 in HEC1B cells treated or untreated with virus and M059J DNA-PK - and M059J cells with DNA-PK+ (Karpova, A.Y., et al. (2002). PNAS. 99(5):2818-2823).
Western Blotting Analysis: A representative lot detected IRF3 in HFK (human foreskin keratinocytes) cells expressing BPV ORF′s (Ronco, L.V., et al. (1998) Genes Dev. 12:2061-2072).
Western Blotting Analysis: A representative lot detected IRF3 in Transfected Mock and SeV infected 293 cells (Basler, C.F., et al. (2003) Journal of Virology. 77(14):7945-7956).
Immunocytochemistry Analysis: A representative lot detected IRF3 in hec-1b cells infected with delNS1 or PR8 (Talon. J., et al. (2000). Journal of Virology. 74(17):7989-7996).
Immunocytochemistry Analysis: A representative lot detected IRF3 in HEC1B cells treated or untreated with virus (Karpova, A.Y., et al. (2002). PNAS. 99(5):2818-2823).

Research Sub CategoryImmunoglobulins & Immunology

This Anti-IRF3 Antibody, clone SL-12 is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of IRF3.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

Immunogen

Recombinant protein corresponding to human IRF3.

Linkage

Replaces: MABF21

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected IRF3 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~49 kDa observed. IRF3 has isoforms at ~49 kDa, ~47 kDa, ~33 kDa, ~16 kDa, & ~12 kDa